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When plain water isn't enough to replenish lost minerals, a medical-grade oral electrolyte solution can rapidly restore cellular balance to stop muscle aches, leg cramps, and tension headaches without the heavy sugars that trigger nausea.
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A buffer consisting of 20 mM HEPES, 100 mM NaCl, pH 7.4, 10 mM MgCl 2 , 1 mM MnCl 2 and 10% glycerol was used to re-suspend the collected membranes