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After heat inactivation of the Proteinase K, samples were used for genotyping with the following primers: Ubiad1 +/− FW 5′-CACGGGGCCGGCAATTTGGTCAAG-3′, RV 5′-GGGGGTCTCCAGCCCCACAGTAAGAGA-3′ and Neo-Cassette 5′-CAGCTCGGGCTCGACTAGAG CTTGC-3′ (400 bp KO allele and 500 bp WT allele)